NeoDisc Download

Please download each file individually. The Manual, Resources, and MD5 checksums are available without registration and can be downloaded through the web browser or using command-line download managers (e.g., using wget).

NeoDisc software requires email registration. After registering, you will receive an email with a unique download link. Please ensure that you open the link in the same browser used for registration and that cookies are enabled on the website.

Version 1.7.0 – bugfix 2

Bug Fixes:

• Improvement in the -cleandnafq and -cleanrnafq outputs: support for a wider range of FASTQ sequence identifier formats.

• Addition of light and full modes to -cleandnafq and -cleanrnafq:

light mode reformats FASTQ read identifiers into a format compatible with GATK and Picard tools.

full mode performs the same reformatting and also deduplicates reads.

• Fixed a bug affecting the combination of MS-identified peptides when only HLA-I or HLA-II injections were measured, but predicted binders existed for both HLA-I and HLA-II.

Version 1.7.0 – bugfix 1

To enhance the download experience, the resources have been separated from the container. As a result, the installation process has changed slightly, as reflected in the updated Manual. Additionally, a few bugs have been fixed in the Software and Resources.

Bug Fixes:

• Fixed issue with running MS analysis using DIA only.

• Resolved problem when running MS analysis with only HLA-I or HLA-II alleles.

• Corrected underestimation of mutational load when using gencode_v43 as the capture kit.

• Added –no-home and –cleanenv options to the Singularity command to prevent loading environment variables in the container.

• Various minor bug fixes.

Version 1.7.0

First release of NeoDisc, as published in:

A comprehensive proteogenomic pipeline for neoantigen discovery to advance personalized cancer immunotherapy
Huber F, et al. Nature Biotechnology, 2024, 10.1038/s41587-024-02420-y